SupplySide West Expo 2011

By Mike Gottschalk

Laszlo Torma inside the Venetian Hotel & Casino

The Supplyside West Conference is a very important Herbal Supplement Industry event. It provides important educational and networking opportunities to this $29 billion market. Since the FDA set out required current good manufacturing practices (cGMP) in 2007, the Herbal Supplement producers are working to meet the new requirements before the FDA audits. These regulations are intended to insure the quality, purity and safety of the Herbal Supplement products available today.  

The show is held in Las Vegas, Nevada, October 19-23. Pickering Laboratories exhibited at the show. We introduced our instruments, and methods to the industry. Of primary concern are Mycotoxins and Amino Acids, but also pesticides. Our GPC Clean-up and post-column instruments are well-suited to these applications. 

In addition to the literature and instruments in the booth, we presented two posters:

“Immunoaffinity Clean-up and  Analysis of Aflatoxins and Ochratoxin A in Herbs & Spices using HPLC with Post-column Photochemical Derivatization”

The production of herbal supplements and spices is a fast growing industry. There are numerous reports on the presence of Mycotoxins in commercially available herbs and spices such as chamomile, black and white tea leaves, ginkgo leaves, paprika and cumin. We developed a simple, sensitive and robust HPLC-Post Column Derivatization method for analyzing Aflatoxins B1, B2, G1, G2 and Ochratoxin A in herbs and spices. AflaOTAClean Immunoaffinity columns contain antibodies specific for both classes of Mycotoxins and allow for fast and efficient sample clean-up. We used the AcceClean automated workstation, which processes three samples simultaneously.

"Accelerated Amino Acids using HPLC with Post-Column Derivatization"

We developed an accelerated amino acids analysis method for Physiological and Protein Hydrolysate samples that utilizes temperature and eluant gradients. Protein Hydrolysates can be analyzed in 48min (inject-to-inject). Amino Acid Analysis is an important step in the Quality Control and R&D of dietary supplements, sports drinks, pharmaceuticals and in any type of sample where the amino acid content is of importance. The objective of the study was to reduce the total run time of Amino Acid Analysis by implementing both a column temperature gradient and a linear buffer gradient using HPLC with Post-column derivatization.

If you would like a copy of either or both of these posters or any additional information  about our products, please feel free to send us an email or give us a call:

Email: sales@pickeringlabs.com

Phone: Toll-free: 800-654-3330 Direct: 650-694-6700

Dinner on the canal in "Venice"

Herbal Remedy

By Michael Pickering

Echinacea purpurea, often called a coneflower, has long been used to ameliorate the symptoms of upper respiratory problems caused by viruses and allergies.  The most common form available in the San Francisco Bay Area is in a tablet called Airborne®.  My wife and daughters always have it on hand.  However, if I ingest a tablet, it worsens my symptoms.  I reasoned that the effect was due to the residual incorporated plant material aggravating my hay fever.  So I decided to test the theory by making a tincture wherein the plant material could be categorically removed. 

When water is the extraction solvent, the product is called an infusion and is taken as a hot “tea.”  My solvent of choice for herbaceous tinctures is potable ethanol (190 proof), although as low as 60 proof may be used.  The Everclear® available in California is only 150 proof.  The same brand in Oregon and Nevada is 190 proof, so I had my Oregonian daughter get me some.  That allowed me to filter out the plant residue.  The experiment provided a useable form of Echinacea that is free of allergic side-effects.  My recommended dose is one teaspoon.  I share the extract with my coworkers, friends, and relatives, who all acclaim its efficacy.  One friend actually claims to be allergy-symptom free.

The flower is an annual which my wife and I cultivate in our garden.  Our two plants provide enough cones per season to make 2 liters of solution.  At Pickering Labs, we recently purchased some Echinacea from an herbal supply vendor as a sample for a multi-mycotoxin study, and rather than cones it was supplied as the plant root, called Black Sampson Root. 

Herbal Tincture Preparation:

-          Put 100 g dried herbs into a sufficiently sized jar.

-          Add 500 mL of 190 proof alcohol.

-          Seal, and place on shaker for three days.

o       Note: Longer contact time is recommended for lower proof alcohol.  Two weeks is recommended for 60 proof, for example.

-          Filter using a 0.45 μm filter.

Echinacea purpurea

Pickering Laboratories Introduces Key Products to Herbal Supplements Market

By Mike Gottschalk

The Supplyside West Conference in Las Vegas, Nevada October 19-23, is a very important Herbal Supplement Industry event. It provides important educational and networking opportunities to this $29 billion market. Since the FDA sent out required current good manufacturing practices (cGMP) in 2007, the Herbal Supplement producers are working to meet the new requirements before the FDA audits. These regulations are intended to insure the quality, purity and safety of the Herbal Supplement products available today. Pickering Laboratories had a booth at the show to assist the producers and laboratories with the instrumentation, chemistry, methods, and support for the analysis and clean-up of samples for Amino Acids, Aflatoxins, Carbamate pesticides, Glyphosate Herbicides, and 23 other methods.

Click on the links to see our Latest Method Abstracts, introduced at the show:
1) MA 215: Clean-up and Determination of Alfatoxins in Peanuts and Peanut Butter 

2) MA 218: Clean-up and Analysis of Aflatoxins and Ochratoxin A in Herbs and Spices 


The annual meeting of the AOAC International in Orlando, Florida on September 20-25, also had a strong emphasis on Herbal Supplements testing. The AOAC organization is central to FDA efforts to provide analytical structure to testing methods for this newly regulated industry. Pickering Laboratories participated in the AOAC sponsored Single Laboratory Validation of Multi-residue Mycotoxin Analysis in Corn. For this work, Pickering Laboratories was awarded the “Single Lab Validation of the Year”. This pivotal work in the analysis of Mycotoxins continues in Herbal Supplements as well as agricultural products.

Send us an email to support@pickeringlabs.com to request a copy of our poster: “Clean-up and Determination of Alfatoxins in Peanuts and Peanut” from the AOAC show.




Our next show is Pittcon 2011 on March 13-18 in Atlanta, Georgia.

See you there!

NIST/NIH Dietary Supplements Quality Assurance Program Workshop

By Wendy Rasmussen

Natural products industry is under increasing pressure to increase analytical testing of raw materials and finished products. This not only includes contaminants (such as heavy metals, pesticides, and mycotoxins), but also identification and nutrition labeling.

The DSHEA (Dietary Supplement Health & Education Act) created the Office of Dietary Supplements in 2002. The Dietary Supplement Quality Assurance Program, is a collaboration between the NIH's ODS and the NIST. The program consists of Exercises in which several samples are sent out to participating labs throughout the year, and the results are then analyzed in a report. This is a completely voluntary exercise, and it gives a laboratory a great opportunity to check their results against a certified value and also against those of their peers. Even though participation is not part of a regulatory certification, the choice to participate greatly increases confidence and credibility.

And since the NIST are working closely with the NIH's Office of Dietary Supplements, I asked the ODS if they could describe the relationship:

"The DSQAP is part of the ODS Analytical Methods and Reference Materials Program. We’ve been funding them to produce supplement matrix reference materials since about 2002. A few years ago, we discovered that NIST had QA programs for other commodities, and we thought that a dietary supplement program would complement the reference materials already being produced and added funding to the inter agency agreement we already had in place with them. While we are a funder for the NIST program, it could be described as more of a collaboration than a strict funding relationship. NIST pretty much matches our funding with internal funding. "

The NIST website http://www.nist.gov/mml/analytical/dsqaprogram.cfm also does a good job of describing the program in more detail. If you would like to participate in the DSLQAP, they would love to have more participants, just send them an email to the address on the above webpage.

This past September, the NIST hosted a workshop in Bethesda, MD which brought together participants from several sample exercises. This was a great opportunity for labs and companies to come together to discuss and learn. Pickering Laboratories participated in the most recent Exercises by analyzing Aflatoxin in peanut samples.

We used our Photochemical reactor (UVE) and our AflaCLEAN Immunoaffinity columns for the sample cleanup. The results can be found on our new application note and in a presentation given to the group at the workshop.

We gave a quick presentation to the group on Thursday morning, detailing our method and results. We would love to share our talk titled simply  "Aflatoxin Analysis". Just send us an email to support@pickeringlabs.com and we'll send you a copy!

The Photochemical derivatization of Aflatoxins has been shown to be a very rugged and sensitive method for analyzing low-levels of the toxins. And the Immunoaffinity columns can be used to cleanup a very wide variety of sample matrices. Indeed, there have been several publications showing the results using Photochemical Derivatization.

We are now distributing Mycotoxin Immunoaffinity products for Ochratoxin and Aflatoxin. The performance and batch-to-batch reproducibility of the columns is exceptional and far exceeds that of other manufacturers. The columns can be used for any matrix, from wine and juice, to nuts and grains, to herbs and spices. Contact Sales for more information.

Definition of Acronyms:
NIH - National Institutes of Health
ODS - Office of Dietary Supplements, part of NIH
NIST - National Institute of Standards and Technology
DSLQAP - Dietary Supplement Laboratory Quality Assurance Program, formed by the ODS in collaboration with NIST
AHPA - American Herbal Products Association
AOAC - Association of Analytical Chemists

Laszlo Torma has also contributed a very nice piece about our Membership in the AHPA on our blog.

PICKERING LABORATORIES IS AHPA MEMBER, by Laszlo Torma

This year we joined the American Herbal Products Association (AHPA) and I was appointed to the joint Botanical Raw Materials, Standards and Analytical Labs committee. AHPA is a national trade organization and it is focusing on promoting the responsible commerce of herbal products.

AHPA is also involved with key scientists and organizations that conduct research on herbs and maintain an active role with the various standards –setting organizations.

AHPA and AOACI are working together to establish and validate analytical methods for herbal products.

I attended the AHPA meeting in California for the first time in March. They are a very active and interesting association. They promote self-regulation, crisis management, education and other areas. They usually meet jointly with Natural Products Expo (East and West) which is a private, very large trade show.

AHPA also encourages member and contract laboratories to participate in the National Institute of Standards and Technology (NIST) Dietary Supplement Laboratory Quality Assurance Program. This program allows laboratories to perform specific analyses and to see their results against known values. Pickering Laboratories is participating in this

Quality Assurance Program in the area of analysis of aflatoxins in peanut products.